Hotspot

accD-psaI

LSC · 60213-60881 · 669 bp

Back to Phytolacca

Pi 0.0251

MarkerSeek score 59.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 669
variable_sites: 42
pi: 0.0251
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GAGTTATTTCAGCTGCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	624-796 bp	1.0	87.6
accD-psaI_p2	GAGTTATTTCAGCTGCACGC	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.8	57.9	630-802 bp	1.0	87.0
accD-psaI_p3	GTGAGTTATTTCAGCTGCACG	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.9	58.0	626-798 bp	1.0	80.1
accD-psaI_p4	GTGAGTTATTTCAGCTGCACG	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.9	57.9	632-804 bp	1.0	79.4
accD-psaI_p5	GAGTTATTTCAGCTGCACGC	CCGGAAATACTAGGCCCACT	50.0	55.0	57.8	58.9	612-784 bp	0.909091	75.8