Hotspot

trnT-UGU-trnL-UAA

LSC · 47135-47878 · 744 bp

Back to Phytolacca

Pi 0.0273

MarkerSeek score 63.90

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 744
variable_sites: 49
pi: 0.0273
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	697-805 bp	1.0	86.1
trnT-UGU-trnL-UAA_p2	GAGTCGAACCGATGACCATC	CCGTAGCGTCTACCGATTTC	55.0	55.0	58.2	58.2	728-836 bp	1.0	85.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	691-799 bp	1.0	84.9
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	CGTCTACCGATTTCGCCATA	55.0	50.0	58.2	57.6	722-830 bp	1.0	84.5
trnT-UGU-trnL-UAA_p5	CTAACCTCTGAGCTAAGCGG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.5	58.2	689-797 bp	1.0	83.2