Hotspot

atpH-atpI

LSC · 13867-14582 · 716 bp

Back to Phytolacca

Pi 0.0146

MarkerSeek score 61.10

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 716
variable_sites: 28
pi: 0.0146
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	766-792 bp	1.0	82.9
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	822-848 bp	1.0	82.0
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	45.0	55.0	57.9	57.8	782-808 bp	1.0	82.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	767-793 bp	1.0	81.8
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	CGAATCCATGGAGGGTCATC	55.0	55.0	58.2	57.8	826-852 bp	1.0	81.4