Hotspot

trnS-GCU-trnG-UCC

LSC · 8370-9179 · 810 bp

Back to Phytolacca

Pi 0.0307

MarkerSeek score 65.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 59
pi: 0.0307
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CTTTCGTCCACTCAGCCATC	CGGAATAGTAATCAAACCGGGA	55.0	45.5	58.6	57.9	878-920 bp	1.0	67.6
trnS-GCU-trnG-UCC_p2	CTTTCGTCCACTCAGCCATC	TCGGAATAGTAATCAAACCGGG	55.0	45.5	58.6	57.9	879-921 bp	1.0	67.6
trnS-GCU-trnG-UCC_p3	TTTCGTCCACTCAGCCATCT	CGGAATAGTAATCAAACCGGGA	50.0	45.5	59.0	57.9	877-919 bp	1.0	64.5
trnS-GCU-trnG-UCC_p4	TTTCGTCCACTCAGCCATCT	TCGGAATAGTAATCAAACCGGG	50.0	45.5	59.0	57.9	878-920 bp	1.0	64.5
trnS-GCU-trnG-UCC_p5	CTTTCGTCCACTCAGCCATC	CGGAATAGTAATCAAACCGGG	55.0	47.6	58.6	56.5	878-920 bp	1.0	64.1