Hotspot

rps16-trnQ-UUG

LSC · 6110-6886 · 777 bp

Back to Phytolacca

Pi 0.0427
MarkerSeek score 80.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
777
variable_sites
80
pi
0.0427
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TATGTCCTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 536-920 bp 1.0 90.8
rps16-trnQ-UUG_p2 TATGTCCTTCAAGTCGCACG TAAGGCATCGGGTTTTGGTC 50.0 50.0 58.0 58.2 566-950 bp 1.0 89.8
rps16-trnQ-UUG_p3 GCACGTTGCTTTTTACCACA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 521-905 bp 1.0 89.5
rps16-trnQ-UUG_p4 GCACGTTGCTTTTTACCACA TAAGGCATCGGGTTTTGGTC 45.0 50.0 57.8 58.2 551-935 bp 1.0 88.4
rps16-trnQ-UUG_p5 AGTCGCACGTTGCTTTTTAC GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.6 58.1 525-909 bp 1.0 88.0