Hotspot

trnK-UUU-rps16

LSC · 4334-4949 · 616 bp

Back to Phytolacca

Pi 0.0156

MarkerSeek score 66.00

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 27
pi: 0.0156
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	ACGAATCCCTTGCTTCATCC	GGAGGGCAGGAGTTTTGAAA	50.0	50.0	58.0	58.0	704-752 bp	1.0	86.7
trnK-UUU-rps16_p2	ACGAATCCCTTGCTTCATCC	GCTCAACCTACAGAAACCGT	50.0	50.0	58.0	57.8	738-786 bp	1.0	85.3
trnK-UUU-rps16_p3	TCCTAGCCGCACTTAAAAGC	GGAGGGCAGGAGTTTTGAAA	50.0	50.0	58.3	58.0	670-718 bp	1.0	85.0
trnK-UUU-rps16_p4	GAATCCCTTGCTTCATCCGA	GGAGGGCAGGAGTTTTGAAA	50.0	50.0	57.7	58.0	702-750 bp	1.0	84.4
trnK-UUU-rps16_p5	TCCTAGCCGCACTTAAAAGC	GCTCAACCTACAGAAACCGT	50.0	50.0	58.3	57.8	704-752 bp	1.0	83.6