Hotspot

ndhF-rpl32

SSC · 114245-115450 · 1206 bp

Back to Physospermopsis

Pi 0.0262
MarkerSeek score 65.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1206
variable_sites
82
pi
0.0262
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCATATTGATATGTCTGTTCCA CCAAATATTTTTACGAATACGCT 36.4 30.4 54.1 53.9 776-1336 bp 1.0 49.4
ndhF-rpl32_p2 GCATATTGATATGTCTGTTCCA TCCAAATATTTTTACGAATACGCT 36.4 29.2 54.1 55.3 777-1337 bp 1.0 49.3
ndhF-rpl32_p3 GCATATTGATATGTCTGTTCCA TCCAAATATTTTTACGAATACGCTT 36.4 28.0 54.1 56.0 777-1337 bp 1.0 49.3
ndhF-rpl32_p4 GCATATTGATATGTCTGTTCCA TTCCAAATATTTTTACGAATACGCT 36.4 28.0 54.1 56.0 778-1338 bp 1.0 49.3
ndhF-rpl32_p5 GCATATTGATATGTCTGTTCCA TCCAAATATTTTTACGAATACGC 36.4 30.4 54.1 53.9 777-1337 bp 1.0 49.3