Hotspot

trnE-UUC-trnT-GGU

LSC · 31691-32493 · 803 bp

Back to Physospermopsis

Pi 0.0309

MarkerSeek score 72.10

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 59
pi: 0.0309
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CAATCCCGGGGAAATAAGGG	TGGTACATGAAGCTGCGATC	55.0	50.0	58.0	58.1	1854-1913 bp	1.0	87.8
trnE-UUC-trnT-GGU_p2	CAATCCCGGGGAAATAAGGG	TGGTGATTCCAGAGACGACT	55.0	50.0	58.0	58.1	1427-1479 bp	1.0	87.8
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TGGTACATGAAGCTGCGATC	50.0	50.0	57.8	58.1	1560-1619 bp	1.0	87.5
trnE-UUC-trnT-GGU_p4	CAATCCCGGGGAAATAAGGG	TTTGGTACATGAAGCTGCGA	55.0	45.0	58.0	57.8	1856-1915 bp	1.0	86.8
trnE-UUC-trnT-GGU_p5	CAATCCCGGGGAAATAAGGG	TACCGCTGAGTTAAAAGGGC	55.0	50.0	58.0	57.9	1141-1193 bp	0.75	75.0