Hotspot

psbM-trnD-GUC

LSC · 30601-31280 · 680 bp

Back to Physospermopsis

Pi 0.0179

MarkerSeek score 65.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 35
pi: 0.0179
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCCTTATTTCCCCGGGATTG	45.0	55.0	55.8	58.0	814-825 bp	1.0	67.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TCCCTTATTTCCCCGGGATT	45.0	50.0	55.8	58.1	815-826 bp	1.0	66.5
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	45.0	50.0	55.8	58.3	806-817 bp	1.0	64.5
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	45.0	50.0	55.8	58.3	807-818 bp	1.0	64.5
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	CCCTTATTTCCCCGGGATTG	40.9	55.0	57.4	58.0	821-832 bp	1.0	63.9