Hotspot

atpF-atpH

LSC · 13663-14046 · 384 bp

Back to Physospermopsis

Pi 0.0338

MarkerSeek score 73.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 384
variable_sites: 31
pi: 0.0338
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	58.0	58.2	464-486 bp	1.0	85.3
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	491-513 bp	1.0	84.3
atpF-atpH_p3	CCAGTGACCCAAAGAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	58.0	57.6	466-488 bp	1.0	83.9
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	493-515 bp	1.0	82.9
atpF-atpH_p5	CCAGTGACCCAAAGAAACGA	CGGAGGGAAAAATACGAGGT	50.0	50.0	58.0	57.3	530-552 bp	1.0	80.9