Pi
0.00267
MarkerSeek score
60.50
Primer available
yes
Label
rrn16
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rrn16_p1 | CGAATCCGCTTTGTCTACGA | AACTCAGACGTAGCTCCCTT | 50.0 | 50.0 | 58.1 | 58.1 | 1635 bp | 1.0 | 78.9 |
| rrn16_p2 | CGAATCCGCTTTGTCTACGA | TGTGAAGCAGTGTCAAACCA | 50.0 | 45.0 | 58.1 | 57.9 | 1601 bp | 1.0 | 78.6 |
| rrn16_p3 | GAACTCCGGGCGAATATGAA | AACTCAGACGTAGCTCCCTT | 50.0 | 50.0 | 57.8 | 58.1 | 1696 bp | 1.0 | 77.8 |
| rrn16_p4 | CGAATCCGCTTTGTCTACGA | TCAGACGTAGCTCCCTTCTT | 50.0 | 50.0 | 58.1 | 57.8 | 1632 bp | 1.0 | 77.8 |
| rrn16_p5 | AACTCCGGGCGAATATGAAG | AACTCAGACGTAGCTCCCTT | 50.0 | 50.0 | 57.8 | 58.1 | 1695 bp | 1.0 | 77.8 |
| rrn16_p1 | AACTCAGACGTAGCTCCCTT | CGAATCCGCTTTGTCTACGA | 50.0 | 50.0 | 58.1 | 58.1 | 1635 bp | 1.0 | 78.9 |
| rrn16_p2 | TGTGAAGCAGTGTCAAACCA | CGAATCCGCTTTGTCTACGA | 45.0 | 50.0 | 57.9 | 58.1 | 1601 bp | 1.0 | 78.6 |
| rrn16_p3 | AACTCAGACGTAGCTCCCTT | GAACTCCGGGCGAATATGAA | 50.0 | 50.0 | 58.1 | 57.8 | 1696 bp | 1.0 | 77.8 |
| rrn16_p4 | TCAGACGTAGCTCCCTTCTT | CGAATCCGCTTTGTCTACGA | 50.0 | 50.0 | 57.8 | 58.1 | 1632 bp | 1.0 | 77.8 |
| rrn16_p5 | AACTCAGACGTAGCTCCCTT | AACTCCGGGCGAATATGAAG | 50.0 | 50.0 | 58.1 | 57.8 | 1695 bp | 1.0 | 77.8 |