Hotspot

rps16

LSC · 5026-6113 · 1088 bp

Back to Physochlaina

Pi 0.00429

MarkerSeek score 46.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1088
variable_sites: 7
pi: 0.00429
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ACTTGTGTTGGATTGGCACT	TGGGAAGGCGTTCATTTCAA	45.0	45.0	57.9	58.0	2211-2225 bp	1.0	80.2
rps16_p2	ACTTGTGTTGGATTGGCACT	AACGGTGACAATTGCTCAGT	45.0	45.0	57.9	58.0	2183-2197 bp	1.0	80.1
rps16_p3	ACTTGTGTTGGATTGGCACT	TGAGTCACGTGAAGATGCAG	45.0	50.0	57.9	57.9	1766-1779 bp	1.0	79.6
rps16_p4	ACTTGTGTTGGATTGGCACT	CAGTAATGGGAAGGCGTTCA	45.0	50.0	57.9	57.9	2217-2231 bp	1.0	79.5
rps16_p5	AAGCCGAGTACTCTACCGTT	TGGGAAGGCGTTCATTTCAA	50.0	45.0	58.2	58.0	2524-2536 bp	1.0	79.5