Hotspot

ccsA

SSC · 116637-117578 · 942 bp

Back to Physochlaina

Pi 0.00425

MarkerSeek score 47.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 942
variable_sites: 6
pi: 0.00425
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.0	58.0	1723 bp	1.0	80.2
ccsA_p2	TGAAATCGGTAGACACGCTG	TAGCTTTTCGATGGCTTCCC	50.0	50.0	58.0	58.0	1722 bp	1.0	80.2
ccsA_p3	GTGAAATCGGTAGACACGCT	GCTGATTCATACAATGCGCC	50.0	50.0	58.0	58.0	1171 bp	1.0	80.1
ccsA_p4	TGAAATCGGTAGACACGCTG	GCTGATTCATACAATGCGCC	50.0	50.0	58.0	58.0	1170 bp	1.0	80.1
ccsA_p5	GTGAAATCGGTAGACACGCT	TGACGAAATGGGCGGAATAG	50.0	50.0	58.0	58.1	1777 bp	1.0	80.1