Hotspot

accD

LSC · 59179-60693 · 1515 bp

Back to Physochlaina

Pi 0.00264

MarkerSeek score 60.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 6
pi: 0.00264
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCCTAGTGATTGGATTTCTATGCT	TCACCAAAGAAAACGCCATT	37.5	40.0	57.2	56.4	1684 bp	1.0	40.3
accD_p2	TCCTAGTGATTGGATTTCTATGCT	ATCACCAAAGAAAACGCCAT	37.5	40.0	57.2	56.2	1685 bp	1.0	40.3
accD_p3	TCCTAGTGATTGGATTTCTATGC	TCACCAAAGAAAACGCCATT	39.1	40.0	55.9	56.4	1684 bp	1.0	40.3
accD_p4	CCTAGTGATTGGATTTCTATGCT	TCACCAAAGAAAACGCCATT	39.1	40.0	55.9	56.4	1683 bp	1.0	40.3
accD_p5	TCCTAGTGATTGGATTTCTATGCTT	TCACCAAAGAAAACGCCATT	36.0	40.0	57.9	56.4	1684 bp	1.0	40.3