Hotspot

petA-psbJ

LSC · 65177-66250 · 1074 bp

Back to Physochlaina

Pi 0.0109

MarkerSeek score 52.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1074
variable_sites: 17
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCAGAGCTATCGCCCCAATA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2292-2300 bp	1.0	80.7
petA-psbJ_p2	GGACGGCAAGTGGTTGATAT	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2000-2008 bp	1.0	80.6
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	TGCGGGTTGGTTATTTGTCA	55.0	45.0	58.0	58.0	1896-1904 bp	1.0	80.6
petA-psbJ_p4	TCCGATCGTATTTCTCCCGA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2342-2350 bp	1.0	80.5
petA-psbJ_p5	TCAGAGCTATCGCCCCAATA	GAGCCGACAAGGAATTCCAT	50.0	50.0	58.0	58.0	2214-2222 bp	1.0	80.5