Hotspot

atpB-rbcL

LSC · 56163-56935 · 773 bp

Back to Physochlaina

Pi 0.00431

MarkerSeek score 61.60

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 773
variable_sites: 5
pi: 0.00431
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	881-884 bp	1.0	71.7
atpB-rbcL_p2	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	880-883 bp	1.0	71.4
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	CCTTGGTTTGGTACTCAGGAG	55.0	52.4	58.1	57.9	929-932 bp	1.0	71.3
atpB-rbcL_p4	GGAAACCCCAGAACCAGAAG	GTGGTGACATAAATCCCTCCC	55.0	52.4	58.1	58.1	842-845 bp	1.0	71.3
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TGGTGACATAAATCCCTCCCT	55.0	47.6	58.1	58.1	841-844 bp	1.0	70.7