Hotspot

ndhC-trnV-UAC

LSC · 52047-53140 · 1094 bp

Back to Physochlaina

Pi 0.00672

MarkerSeek score 52.10

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1094
variable_sites: 10
pi: 0.00672
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	31.8	50.0	53.9	57.8	1150-1162 bp	1.0	40.9
ndhC-trnV-UAC_p2	TCATATTCGTAAAGCAGAAACA	GAAGGTCTACGGTTCGAGTC	31.8	55.0	53.9	57.5	1149-1161 bp	1.0	40.9
ndhC-trnV-UAC_p3	ATCATATTCGTAAAGCAGAAACA	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1151-1163 bp	1.0	40.9
ndhC-trnV-UAC_p4	TCATATTCGTAAAGCAGAAACAT	AGAAGGTCTACGGTTCGAGT	30.4	50.0	54.5	57.8	1150-1162 bp	1.0	40.9
ndhC-trnV-UAC_p5	TCATATTCGTAAAGCAGAAACA	GTTCGAGTCCGTATAGCCCT	31.8	55.0	53.9	58.7	1138-1150 bp	1.0	40.9