Hotspot

rps16-trnQ-UUG

LSC · 6114-7313 · 1200 bp

Back to Physochlaina

Pi 0.00889

MarkerSeek score 64.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1200
variable_sites: 16
pi: 0.00889
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1290-1312 bp	1.0	79.8
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1273-1295 bp	1.0	79.3
rps16-trnQ-UUG_p3	CAACGGATCGTGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1291-1313 bp	1.0	78.8
rps16-trnQ-UUG_p4	CGGATCGTGTCCTTCAAGTC	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1288-1310 bp	1.0	77.5
rps16-trnQ-UUG_p5	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1287-1309 bp	1.0	77.5