Hotspot

rps16

LSC · 5319-6476 · 1158 bp

Back to Physocarpus

Pi 0.000864

MarkerSeek score 59.20

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1158
variable_sites: 1
pi: 0.000864
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGAGGAAATAGCAAAGAACCA	TATGTACCGACCGATCCGAT	38.1	50.0	54.8	57.8	1308-1309 bp	1.0	45.1
rps16_p2	AAGAGGAAATAGCAAAGAACCA	TATGTACCGACCGATCCGAT	36.4	50.0	55.6	57.8	1309-1310 bp	1.0	43.5
rps16_p3	AGAGGAAATAGCAAAGAACCAA	TATGTACCGACCGATCCGAT	36.4	50.0	55.6	57.8	1308-1309 bp	1.0	43.5
rps16_p4	AGAGGAAATAGCAAAGAACCA	ACCGACCGATCCGATGATTA	38.1	50.0	54.8	58.4	1303-1304 bp	1.0	43.5
rps16_p5	AGAGGAAATAGCAAAGAACCA	TACCGACCGATCCGATGATT	38.1	50.0	54.8	58.4	1304-1305 bp	1.0	43.5