Hotspot

rpl33

LSC · 70781-70981 · 201 bp

Back to Physocarpus

Pi 0

MarkerSeek score 23.20

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 201
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 23.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	CCTTGCTTCGGATCGGAAAA	CCTTGGAAGACTGACACACG	50.0	55.0	58.6	58.5	293 bp	1.0	71.6
rpl33_p2	CCTTGCTTCGGATCGGAAAA	CTTGGAAGACTGACACACGA	50.0	50.0	58.6	57.5	292 bp	1.0	71.5
rpl33_p3	CCTTGCTTCGGATCGGAAAA	TTGGAAGACTGACACACGAG	50.0	50.0	58.6	57.5	291 bp	1.0	71.5
rpl33_p4	CCTTGCTTCGGATCGGAAAA	TCCTTGGAAGACTGACACAC	50.0	50.0	58.6	57.1	294 bp	1.0	68.3
rpl33_p5	CCTTGCTTCGGATCGGAAA	CCTTGGAAGACTGACACACG	52.6	55.0	57.8	58.5	293 bp	1.0	66.8