Hotspot

rpl16

LSC · 84553-85946 · 1394 bp

Back to Physocarpus

Pi 0.000717

MarkerSeek score 59.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1394
variable_sites: 1
pi: 0.000717
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCAATGCAAAGCAAAGGTCT	ACAACAAATGGCAAACTCTTTCA	40.0	34.8	56.1	57.9	1620 bp	1.0	40.4
rpl16_p2	TCAATGCAAAGCAAAGGTCT	TGGTGATAAAACAACAAATGGCA	40.0	34.8	56.1	57.7	1630 bp	1.0	40.0
rpl16_p3	TTCAATGCAAAGCAAAGGTCT	ACAACAAATGGCAAACTCTTTCA	38.1	34.8	56.9	57.9	1621 bp	1.0	40.0
rpl16_p4	TTCAATGCAAAGCAAAGGTCT	TGGTGATAAAACAACAAATGGCA	38.1	34.8	56.9	57.7	1631 bp	1.0	40.0
rpl16_p5	TTTCAATGCAAAGCAAAGGTCT	ACAACAAATGGCAAACTCTTTCA	36.4	34.8	57.6	57.9	1622 bp	1.0	40.0