Hotspot

psbD

LSC · 34953-36014 · 1062 bp

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Pi 0.000942

MarkerSeek score 59.30

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 1
pi: 0.000942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	AAAAGAAGGGGCAGTGTACG	ACGACCGGCTAAAGCTAAAG	50.0	50.0	58.1	58.0	1205 bp	1.0	79.2
psbD_p2	AAAAGAAGGGGCAGTGTACG	CGACCGGCTAAAGCTAAAGT	50.0	50.0	58.1	58.0	1204 bp	1.0	79.2
psbD_p3	AAAAGAAGGGGCAGTGTACG	GAGCACCCAGTAGTTTACCG	50.0	55.0	58.1	58.0	1284 bp	1.0	79.2
psbD_p4	GGGGCAGTGTACGAGAAATC	ACGACCGGCTAAAGCTAAAG	55.0	50.0	58.3	58.0	1198 bp	1.0	77.2
psbD_p5	GGGGCAGTGTACGAGAAATC	CGACCGGCTAAAGCTAAAGT	55.0	50.0	58.3	58.0	1197 bp	1.0	77.2