Hotspot

atpA

LSC · 10241-11764 · 1524 bp

Back to Physocarpus

Pi 0.000656

MarkerSeek score 59.10

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 1
pi: 0.000656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	TGATGAAGATTCAAAGTAATCACT	TAGTCCTTCTACTGCAGGCA	29.2	50.0	54.3	57.8	1606-1607 bp	1.0	40.0
atpA_p2	TGATGAAGATTCAAAGTAATCACT	GTCCTTCTACTGCAGGCATT	29.2	50.0	54.3	57.6	1604-1605 bp	1.0	40.0
atpA_p3	TGATGAAGATTCAAAGTAATCACT	AGTCCTTCTACTGCAGGCAT	29.2	50.0	54.3	58.4	1605-1606 bp	1.0	40.0
atpA_p4	TTGATGAAGATTCAAAGTAATCACT	TAGTCCTTCTACTGCAGGCA	28.0	50.0	55.0	57.8	1607-1608 bp	1.0	40.0
atpA_p5	TGATGAAGATTCAAAGTAATCAC	TAGTCCTTCTACTGCAGGCA	30.4	50.0	52.8	57.8	1606-1607 bp	1.0	40.0