Hotspot

ndhF-rpl32

SSC · 116158-117224 · 1067 bp

Back to Physocarpus

Pi 0.000945

MarkerSeek score 60.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1067
variable_sites: 1
pi: 0.000945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCCGTTGGAAAAAGT	TTCAATGCTGCCCGATATCC	40.0	50.0	57.0	58.1	1247-1256 bp	1.0	71.5
ndhF-rpl32_p2	TTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	40.0	45.0	57.0	57.8	1254-1263 bp	1.0	70.9
ndhF-rpl32_p3	TTTGTTGCCGTTGGAAAAAGT	TTCAATGCTGCCCGATATCC	38.1	50.0	57.7	58.1	1248-1257 bp	1.0	69.2
ndhF-rpl32_p4	TTTGTTGCCGTTGGAAAAAGT	AAAAGCTTTCAATGCTGCCC	38.1	45.0	57.7	57.8	1255-1264 bp	1.0	68.7
ndhF-rpl32_p5	TTGTTGCCGTTGGAAAAAGT	TGCCCGATATCCCTTCCTTT	40.0	50.0	57.0	58.5	1239-1248 bp	1.0	68.5