Hotspot

rpl20-rps12

LSC · 72112-72899 · 788 bp

Back to Physocarpus

Pi 0.00381

MarkerSeek score 60.90

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 788
variable_sites: 3
pi: 0.00381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	944-951 bp	1.0	75.0
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	943-950 bp	1.0	75.0
rpl20-rps12_p3	TGTTCTACGTCTTCGGGCTA	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	900-907 bp	1.0	74.5
rpl20-rps12_p4	TTTGTTCTACGTCTTCGGGC	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	902-909 bp	1.0	74.2
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	CAGCGACGAGGAACATGTAC	50.0	55.0	57.9	58.7	906-913 bp	1.0	73.5