Hotspot

petA-psbJ

LSC · 65879-66839 · 961 bp

Back to Physocarpus

Pi 0.00104

MarkerSeek score 59.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGCTCTTCTTGGCATCTGTT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1080-1082 bp	1.0	71.6
petA-psbJ_p2	TTGCTCTTCTTGGCATCTGT	TCCGGATTAGGTTCATCCCT	45.0	50.0	57.4	57.5	1081-1083 bp	1.0	71.6
petA-psbJ_p3	TGCTCTTCTTGGCATCTGTT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1079-1081 bp	1.0	71.1
petA-psbJ_p4	TTGCTCTTCTTGGCATCTGT	CCGGATTAGGTTCATCCCTG	45.0	55.0	57.4	57.4	1080-1082 bp	1.0	71.1
petA-psbJ_p5	CGTGTCCAAGGTCTTTTGCT	TCCGGATTAGGTTCATCCCT	50.0	50.0	58.7	57.5	1096-1098 bp	1.0	70.6