Hotspot

rbcL-accD

LSC · 59669-60306 · 638 bp

Back to Physocarpus

Pi 0
MarkerSeek score 63.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
638
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGTCCTGAACTAGCTGCTG CGAATCGAACCGCCATTTTT 55.0 45.0 57.7 57.7 710-738 bp 1.0 78.7
rbcL-accD_p2 GAGTCCTGAACTAGCTGCTG ATCGAATCGAACCGCCATTT 55.0 45.0 57.7 58.3 712-740 bp 1.0 78.1
rbcL-accD_p3 GAGTCCTGAACTAGCTGCTG ATCGAACCGCCATTTTTCCA 55.0 45.0 57.7 58.5 707-735 bp 1.0 77.1
rbcL-accD_p4 GAGTCCTGAACTAGCTGCTG TCGAACCGCCATTTTTCCAT 55.0 45.0 57.7 58.5 706-734 bp 1.0 77.1
rbcL-accD_p5 GCTGCTGCTTGTGAAGTATG CGAATCGAACCGCCATTTTT 50.0 45.0 57.5 57.7 697-725 bp 1.0 76.8