Hotspot

trnF-GAA-ndhJ

LSC · 51425-52167 · 743 bp

Back to Physocarpus

Pi 0

MarkerSeek score 60.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 743
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	912-917 bp	1.0	80.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	876-881 bp	1.0	80.2
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	900-905 bp	1.0	79.5
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	CCACGCCTGAAACGTATCTT	55.0	50.0	58.0	58.3	910-915 bp	1.0	78.8
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCACGCCTGAAACGTATCTT	50.0	50.0	58.1	58.3	874-879 bp	1.0	78.5