Hotspot

rps16

LSC · 4962-6099 · 1138 bp

Back to Physaria

Pi 0.0088

MarkerSeek score 54.10

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1138
variable_sites: 10
pi: 0.0088
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CGAAGCGAAAGAGGAGGAAA	ACTGACTGAACTATGACTATTCATGA	50.0	34.6	57.9	57.7	1309-1310 bp	1.0	40.7
rps16_p2	GATCGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCATGA	55.0	34.6	58.2	57.7	1312-1313 bp	1.0	40.7
rps16_p3	CGAAGCGAAAGAGGAGGAAA	ACTGACTGAACTATGACTATTCA	50.0	34.8	57.9	54.6	1309-1310 bp	1.0	40.7
rps16_p4	GATCGAAGCGAAAGAGGAGG	ACTGACTGAACTATGACTATTCA	55.0	34.8	58.2	54.6	1312-1313 bp	1.0	40.7
rps16_p5	CGAAGCGAAAGAGGAGGAAA	TGAATTACACAAACTAGAGGAATGT	50.0	32.0	57.9	56.5	1270-1271 bp	1.0	40.7