Hotspot

psbA

LSC · 354-1415 · 1062 bp

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Pi 0.00188

MarkerSeek score 49.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AGAAGGCTCATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	56.7	58.4	1168 bp	1.0	66.8
psbA_p2	AAGAAGGCTCATATTGCTCGT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	57.4	58.4	1169 bp	1.0	64.7
psbA_p3	AGAAGGCTCATATTGCTCGTT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	57.4	58.4	1168 bp	1.0	64.7
psbA_p4	AGAAGGCTCATATTGCTCGT	AGAATTTGTGTGCTTGGGAGT	45.0	42.9	56.7	58.1	1171 bp	1.0	61.4
psbA_p5	AGAAGGCTCATATTGCTCGT	TTTGTGTGCTTGGGAGTCC	45.0	52.6	56.7	57.9	1167 bp	1.0	60.9