Hotspot

ndhA

SSC · 119359-121527 · 2169 bp

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Pi 0.00323

MarkerSeek score 44.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2169
variable_sites: 7
pi: 0.00323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CAGCTCGTAGGGTTTGTTGA	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	57.8	58.0	2503-2505 bp	1.0	79.0
ndhA_p2	AGCTCGTAGGGTTTGTTGAC	AGTTGAAGCTCCAAAAGGGG	50.0	50.0	57.8	58.0	2502-2504 bp	1.0	79.0
ndhA_p3	CAGCTCGTAGGGTTTGTTGA	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	57.8	58.0	2502-2504 bp	1.0	79.0
ndhA_p4	AGCTCGTAGGGTTTGTTGAC	GTTGAAGCTCCAAAAGGGGA	50.0	50.0	57.8	58.0	2501-2503 bp	1.0	79.0
ndhA_p5	CAGCTCGTAGGGTTTGTTGA	GGTTTATCCGGACCAATGCT	50.0	50.0	57.8	57.9	2838-2840 bp	1.0	78.6