Hotspot

rpl20-rps12

LSC · 67852-68627 · 776 bp

Back to Physaria

Pi 0.00515

MarkerSeek score 61.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 776
variable_sites: 4
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	ACAGCCAATCCGAAATGTCA	50.0	45.0	58.0	57.8	889-894 bp	1.0	78.5
rpl20-rps12_p2	TTTTGTTCTACGCCTCCGAG	ACAGCCAATCCGAAATGTCA	50.0	45.0	57.9	57.8	903-908 bp	1.0	78.2
rpl20-rps12_p3	TCCGAGCTATATATCCGCGT	ACAAGACAGCCAATCCGAAA	50.0	45.0	58.0	57.7	894-899 bp	1.0	77.9
rpl20-rps12_p4	TCCGAGCTATATATCCGCGT	GAGGAACATGTACTCGGGTG	50.0	55.0	58.0	57.7	833-838 bp	1.0	77.7
rpl20-rps12_p5	TTTTGTTCTACGCCTCCGAG	ACAAGACAGCCAATCCGAAA	50.0	45.0	57.9	57.7	908-913 bp	1.0	77.6