Hotspot

trnM-atpE

LSC · 51677-51815 · 139 bp

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Pi 0.0382

MarkerSeek score 78.80

Primer available yes

Label trnM-atpE

Feature Metadata

label_name: trnM-atpE
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 5
pi: 0.0382
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-atpE_p1	GGCAGGAGTCATTGGTTCAA	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.1	58.0	192-199 bp	1.0	84.3
trnM-atpE_p2	GGCAGGAGTCATTGGTTCAA	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.1	58.0	195-202 bp	1.0	84.2
trnM-atpE_p3	TTGCTTTCATACGGCAGGAG	AGTCGAGGCTCTCAATACGA	50.0	50.0	58.3	58.0	204-211 bp	1.0	82.6
trnM-atpE_p4	TTGCTTTCATACGGCAGGAG	ACGAGTCGAGGCTCTCAATA	50.0	50.0	58.3	58.0	207-214 bp	1.0	82.5
trnM-atpE_p5	CATACGGCAGGAGTCATTGG	AGTCGAGGCTCTCAATACGA	55.0	50.0	58.4	58.0	197-204 bp	1.0	81.6