Hotspot

psbZ-trnG

LSC · 35635-36126 · 492 bp

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Pi 0.00423

MarkerSeek score 62.70

Primer available yes

Label psbZ-trnG

Feature Metadata

label_name: psbZ-trnG
feature_type: igs
region: LSC
length_bp: 492
variable_sites: 2
pi: 0.00423
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG_p1	TCCTGATGGTTGGTCGAGTA	CGGTCAACTATGCCGAATCT	50.0	50.0	57.8	57.8	695-714 bp	1.0	78.8
psbZ-trnG_p2	CCGTTGTATTTGCGTCTCCT	CGGTCAACTATGCCGAATCT	50.0	50.0	58.3	57.8	711-730 bp	1.0	78.3
psbZ-trnG_p3	TTTGCGTCTCCTGATGGTTG	CGGTCAACTATGCCGAATCT	50.0	50.0	58.5	57.8	703-722 bp	1.0	76.7
psbZ-trnG_p4	ATTTGCGTCTCCTGATGGTT	CGGTCAACTATGCCGAATCT	45.0	50.0	57.5	57.8	704-723 bp	1.0	76.6
psbZ-trnG_p5	TCCTGATGGTTGGTCGAGTA	CCGGTCAACTATGCCGAATC	50.0	55.0	57.8	58.8	696-715 bp	1.0	74.3