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psbM-trnD

LSC · 28667-29688 · 1022 bp

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Pi 0.00196

MarkerSeek score 58.30

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1022
variable_sites: 2
pi: 0.00196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGAACAAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.9	58.5	1139-1140 bp	1.0	59.7
psbM-trnD_p2	GGAACAAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.6	58.5	1138-1139 bp	1.0	57.1
psbM-trnD_p3	GGAACAAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	42.9	50.0	56.5	58.5	1138-1139 bp	1.0	56.0
psbM-trnD_p4	CAAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.5	50.0	57.4	58.5	1134-1135 bp	1.0	55.6
psbM-trnD_p5	AGGAACAAGAATGAAGAGTGCA	GTTCAATTGGTCAGAGCACC	40.9	50.0	57.9	57.0	1140-1141 bp	1.0	55.5