Hotspot

ndhF-rpl32

SSC · 114893-115678 · 786 bp

Back to Physalis

Pi 0.0034

MarkerSeek score 58.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 786
variable_sites: 12
pi: 0.0034
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GAATAAGTCCCGCGCCTATT	TGCTACCCAATATCCCTTCCT	50.0	47.6	57.8	57.9	942-952 bp	1.0	72.2
ndhF-rpl32_p2	CGGCGAAAACTTTTTGTTGC	TGCTACCCAATATCCCTTCCT	45.0	47.6	57.7	57.9	973-983 bp	1.0	70.7
ndhF-rpl32_p3	GCGAAAACTTTTTGTTGCCG	TGCTACCCAATATCCCTTCCT	45.0	47.6	57.7	57.9	971-981 bp	1.0	70.7
ndhF-rpl32_p4	GTCCCGCGCCTATTAACATA	TGCTACCCAATATCCCTTCCT	50.0	47.6	57.6	57.9	936-946 bp	1.0	70.0
ndhF-rpl32_p5	GAATAAGTCCCGCGCCTATT	TGATGTCGAAGTGCGTTTTT	50.0	40.0	57.8	56.6	891-901 bp	1.0	69.4