Hotspot

infA-rps8

LSC · 82929-83045 · 117 bp

Back to Physalis

Pi 0.00651

MarkerSeek score 54.70

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 4
pi: 0.00651
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	AGACCGTTGGTAAGCGATTC	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.0	57.7	220-242 bp	1.0	80.5
infA-rps8_p2	TAAACGAACCCGGAACAGAC	GAGAGGCTCGACTAGAAGGA	50.0	55.0	57.8	57.7	236-258 bp	1.0	79.1
infA-rps8_p3	AGACCGTTGGTAAGCGATTC	TCCGCGAATTTTAGGTGGAA	50.0	45.0	58.0	57.5	285-307 bp	1.0	78.6
infA-rps8_p4	AGACCGTTGGTAAGCGATTC	TTCCGCGAATTTTAGGTGGA	50.0	45.0	58.0	57.5	286-308 bp	1.0	78.6
infA-rps8_p5	CCGTTGGTAAGCGATTCAGT	GAGAGGCTCGACTAGAAGGA	50.0	55.0	58.3	57.7	217-239 bp	1.0	78.4