Hotspot

petD-rpoA

LSC · 80716-80887 · 172 bp

Back to Physalis

Pi 0.0141

MarkerSeek score 54.70

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 15
pi: 0.0141
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AGCGACGACTGTCTTTTTGA	TGAACATTTTCGTAGCGAAGA	45.0	38.1	57.8	55.9	360-361 bp	1.0	57.4
petD-rpoA_p2	CAGTAGCGACGACTGTCTTT	TGAACATTTTCGTAGCGAAGA	50.0	38.1	57.7	55.9	364-365 bp	1.0	56.4
petD-rpoA_p3	AGCGACGACTGTCTTTTTGA	TTGAACATTTTCGTAGCGAAGA	45.0	36.4	57.8	56.7	361-362 bp	1.0	55.2
petD-rpoA_p4	TTTGATTGGTACCGCAGTCG	TGAACATTTTCGTAGCGAAGA	50.0	38.1	58.6	55.9	345-346 bp	1.0	54.8
petD-rpoA_p5	CAGTAGCGACGACTGTCTTT	TTGAACATTTTCGTAGCGAAGA	50.0	36.4	57.7	56.7	365-366 bp	1.0	54.2