Hotspot

petA-psbJ

LSC · 65710-66780 · 1071 bp

Back to Physalis

Pi 0.00701

MarkerSeek score 66.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1071
variable_sites: 33
pi: 0.00701
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GAGAAGGTTCAATTGGCCGA	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	872-1153 bp	1.0	82.4
petA-psbJ_p2	TCGAGAAGGTTCAATTGGCC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	874-1155 bp	1.0	82.4
petA-psbJ_p3	AGAAGGTTCAATTGGCCGAA	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.7	58.3	871-1152 bp	1.0	81.5
petA-psbJ_p4	GAGAAGGTTCAATTGGCCGA	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	868-1149 bp	1.0	81.5
petA-psbJ_p5	GAGAAGGTTCAATTGGCCGA	TGGTTCATATTCCGGGTTGG	50.0	50.0	58.2	57.6	873-1154 bp	1.0	81.5