Hotspot

trnL-UAA-trnF-GAA

LSC · 49681-50317 · 637 bp

Back to Physalis

Pi 0.0322

MarkerSeek score 81.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 637
variable_sites: 67
pi: 0.0322
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	CAATGCCGGCAACAATGAAA	TTTGAACTGGTGACACGAGG	45.0	50.0	58.2	58.1	692-842 bp	1.0	88.9
trnL-UAA-trnF-GAA_p2	CAATGCCGGCAACAATGAAA	AATCATGTGCCAGGAACCAG	45.0	50.0	58.2	58.2	713-863 bp	1.0	87.9
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	639-789 bp	1.0	87.2
trnL-UAA-trnF-GAA_p4	CAATGCCGGCAACAATGAAA	GTGCCAGGAACCAGATTTGA	45.0	50.0	58.2	58.1	707-857 bp	0.933333	85.2
trnL-UAA-trnF-GAA_p5	CAATGCCGGCAACAATGAAA	CATGTGCCAGGAACCAGATT	45.0	50.0	58.2	58.2	710-860 bp	0.933333	84.7