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atpH-atpI

LSC · 13740-14970 · 1231 bp

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Pi 0.00378

MarkerSeek score 63.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1231
variable_sites: 23
pi: 0.00378
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.0	58.0	1346-1387 bp	1.0	83.0
atpH-atpI_p2	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1429-1470 bp	1.0	81.9
atpH-atpI_p3	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	55.0	50.0	57.9	58.0	1433-1474 bp	1.0	81.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.2	58.0	1347-1388 bp	1.0	81.8
atpH-atpI_p5	GCCAATCCAGCAGCAATAAC	TACCTGTCATGCTCCTTGGA	50.0	50.0	57.8	58.0	1361-1402 bp	1.0	81.5