Hotspot

rps2

LSC · 16163-16873 · 711 bp

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Pi 0

MarkerSeek score 37.20

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	AGTAAGCGATTCCATAAGGGA	TCAGGGTTCCTCGTCTCTTT	42.9	50.0	56.2	58.0	845-849 bp	1.0	58.1
rps2_p2	AGTAAGCGATTCCATAAGGGA	TTCAGGGTTCCTCGTCTCTT	42.9	50.0	56.2	58.0	846-850 bp	1.0	58.1
rps2_p3	AAGTAAGCGATTCCATAAGGGA	TCAGGGTTCCTCGTCTCTTT	40.9	50.0	57.0	58.0	846-850 bp	1.0	56.0
rps2_p4	AAGTAAGCGATTCCATAAGGGA	TTCAGGGTTCCTCGTCTCTT	40.9	50.0	57.0	58.0	847-851 bp	1.0	56.0
rps2_p5	AGTAAGCGATTCCATAAGGGA	TTTCAGTTCAGGGTTCCTCG	42.9	50.0	56.2	57.5	852-856 bp	1.0	53.8