Hotspot

rpl32-trnL-UAG

SSC · 115849-116817 · 969 bp

Back to Physaliastrum

Pi 0.0021

MarkerSeek score 57.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 969
variable_sites: 2
pi: 0.0021
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAGGGATATTGGGTAGCA	TCCTAAGAGCAGCGTGTCTA	47.6	50.0	57.9	57.9	1103-1113 bp	1.0	71.8
rpl32-trnL-UAG_p2	AGGAAGGGATATTGGGTAGCA	TCGAACCGAGATGCATTAGC	47.6	50.0	57.9	58.1	1129-1139 bp	1.0	71.6
rpl32-trnL-UAG_p3	AGGAAGGGATATTGGGTAGCA	AGCAGCGTGTCTACCAATTT	47.6	45.0	57.9	57.8	1096-1106 bp	1.0	71.3
rpl32-trnL-UAG_p4	AGGAAGGGATATTGGGTAGCA	CCTAAGAGCAGCGTGTCTAC	47.6	55.0	57.9	57.8	1102-1112 bp	1.0	71.2
rpl32-trnL-UAG_p5	AGGAAGGGATATTGGGTAGCA	CTAAGAGCAGCGTGTCTACC	47.6	55.0	57.9	57.8	1101-1111 bp	1.0	71.2