Hotspot

rps19-rpl2

IRb · 87053-87122 · 70 bp

Back to Physaliastrum

Pi 0.0286

MarkerSeek score 68.90

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 70
variable_sites: 2
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	GCCCGGGACCAAGTTACTAT	TTGATTCTTCGTCGCCGTAG	55.0	50.0	59.2	58.1	207 bp	1.0	70.7
rps19-rpl2_p2	GCCCGGGACCAAGTTACTAT	CACAACCCCTTGGGGTTATC	55.0	55.0	59.2	58.2	274 bp	1.0	70.0
rps19-rpl2_p3	GCCCGGGACCAAGTTACTAT	TTGGGGTTATCCTGCACTTG	55.0	50.0	59.2	57.8	265 bp	1.0	69.6
rps19-rpl2_p4	GCCCGGGACCAAGTTACTAT	ATTTGATTCTTCGTCGCCGT	55.0	45.0	59.2	58.3	209 bp	1.0	69.2
rps19-rpl2_p5	CCCGGGACCAAGTTACTATT	TTGATTCTTCGTCGCCGTAG	50.0	50.0	56.6	58.1	206 bp	1.0	68.9