Hotspot

trnF-GAA-ndhJ

LSC · 50444-51138 · 695 bp

Back to Physaliastrum

Pi 0

MarkerSeek score 62.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	AATAGCCGGGATAGCTCAGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	828-844 bp	1.0	81.5
trnF-GAA-ndhJ_p2	ATAGCCGGGATAGCTCAGTT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	827-843 bp	1.0	81.5
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	785-801 bp	1.0	81.3
trnF-GAA-ndhJ_p4	GGGACTGCAAATACTCGTGT	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	871-887 bp	1.0	80.7
trnF-GAA-ndhJ_p5	AAATACTCGTGTTCCCAGCC	AGTTGGATAGGATGGCCCTT	50.0	50.0	57.9	58.1	863-879 bp	1.0	80.6