Hotspot

psbM-trnD-GUC

LSC · 30810-31860 · 1051 bp

Back to Physaliastrum

Pi 0.00381

MarkerSeek score 61.00

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1051
variable_sites: 4
pi: 0.00381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.4	58.0	1205-1206 bp	1.0	59.3
psbM-trnD-GUC_p2	AGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	42.9	50.0	56.4	58.1	1206-1207 bp	1.0	58.7
psbM-trnD-GUC_p3	AAGTAGAAAAGCGGTAGGAACT	CCCGTATTTCCCTGGGATTG	40.9	55.0	57.1	58.0	1206-1207 bp	1.0	57.1
psbM-trnD-GUC_p4	AGAAAAGCGGTAGGAACTAGA	CCCGTATTTCCCTGGGATTG	42.9	55.0	56.1	58.0	1202-1203 bp	1.0	57.1
psbM-trnD-GUC_p5	AAGTAGAAAAGCGGTAGGAACT	TCCCGTATTTCCCTGGGATT	40.9	50.0	57.1	58.1	1207-1208 bp	1.0	56.5