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atpH-atpI

LSC · 13954-15182 · 1229 bp

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Pi 0.000817

MarkerSeek score 59.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 1
pi: 0.000817
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TACCTGTCATGCTCCTTGGA	45.0	50.0	58.0	58.0	1360-1365 bp	1.0	79.7
atpH-atpI_p2	CAGCAGTCCCTTGACCAATT	TACCTGTCATGCTCCTTGGA	50.0	50.0	58.1	58.0	1422-1427 bp	1.0	79.3
atpH-atpI_p3	TACCCTCTACAGCTTGACCC	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.1	58.0	1443-1448 bp	1.0	78.8
atpH-atpI_p4	GCGATACCCTCTACAGCTTG	TACCTGTCATGCTCCTTGGA	55.0	50.0	57.9	58.0	1447-1452 bp	1.0	78.8
atpH-atpI_p5	GTCCCTTGACCAATTCCAGG	TACCTGTCATGCTCCTTGGA	55.0	50.0	58.2	58.0	1417-1422 bp	1.0	78.7