Hotspot

ycf3

LSC · 44418-46417 · 2000 bp

Back to Phyllostachys

Pi 0.000704
MarkerSeek score 41.10
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
2000
variable_sites
16
pi
0.000704
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGA 45.0 45.0 57.8 55.7 2137-2145 bp 1.0 61.4
ycf3_p2 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGA 45.0 45.0 57.8 55.7 2136-2144 bp 1.0 61.4
ycf3_p3 ATCAACAACCAAACCACCCA AGAGCATATGGATCGATCGAT 45.0 42.9 57.8 56.3 2137-2145 bp 1.0 57.9
ycf3_p4 TCAACAACCAAACCACCCAT AGAGCATATGGATCGATCGAT 45.0 42.9 57.8 56.3 2136-2144 bp 1.0 57.9
ycf3_p5 GATCAACAACCAAACCACCC AGAGCATATGGATCGATCGA 50.0 45.0 57.2 55.7 2138-2146 bp 1.0 56.3