Hotspot

psbC

LSC · 10467-11888 · 1422 bp

Back to Phyllostachys

Pi 0.000289

MarkerSeek score 41.20

Primer available yes

Label psbC

Feature Metadata

label_name: psbC
feature_type: gene
region: LSC
length_bp: 1422
variable_sites: 3
pi: 0.000289
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC_p1	TTCGTTTCCCAGGAAATCCG	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.2	58.0	1608 bp	1.0	78.6
psbC_p2	GCAGCGGAAGATCCTGAATT	TGGCTCGGCTAGATGGAATA	50.0	50.0	58.3	58.0	1587 bp	1.0	77.5
psbC_p3	TTCGTTTCCCAGGAAATCCG	AACCGAGCCAGAACAGAAAA	50.0	45.0	58.2	57.7	1589 bp	1.0	75.9
psbC_p4	TTCGTTTCCCAGGAAATCCG	ACCGAGCCAGAACAGAAAAA	50.0	45.0	58.2	57.7	1588 bp	1.0	75.9
psbC_p5	TTCGTTTCCCAGGAAATCCG	GGAATAACCGAGCCAGAACA	50.0	50.0	58.2	57.6	1594 bp	1.0	75.5