Hotspot

ndhK

LSC · 51391-52131 · 741 bp

Back to Phyllostachys

Pi 0.000672

MarkerSeek score 40.50

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 741
variable_sites: 1
pi: 0.000672
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	GATAACCAACCCTGCTGCAT	TCTCTACCCTTGGGCAATGA	50.0	50.0	58.2	58.0	979 bp	1.0	77.9
ndhK_p2	ATAACCAACCCTGCTGCATT	TCTCTACCCTTGGGCAATGA	45.0	50.0	57.8	58.0	978 bp	1.0	77.9
ndhK_p3	GATAACCAACCCTGCTGCAT	GGTTCAGTTTATGCATGGCG	50.0	50.0	58.2	57.8	894 bp	1.0	76.7
ndhK_p4	ATAACCAACCCTGCTGCATT	GGTTCAGTTTATGCATGGCG	45.0	50.0	57.8	57.8	893 bp	1.0	76.7
ndhK_p5	GATAACCAACCCTGCTGCAT	CGACGTATTGGGTGTATCCG	50.0	55.0	58.2	58.2	955 bp	1.0	76.3